- GC Women University, Pakistan
- Title:Antibacterial Activity of Extract Formulations of Tachyspermum ammi, Amomum subulatum and Cinnamomum verum
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Emergence of antimicrobial resistance worldwide is responsible for alarming situation. There is an urgent need to discover more effective, safer and less toxic antibacterial agents. Medicinal plants are valuable alternative resources for developing novel drugs. In the present study hexane and acetone extracts of Tachyspermum ammi seeds, Amomum subulatum fruit and Cinnamomum verum bark, independently and in combinations were used to evaluate invitro antibacterial efficacies against four clinical bacterial strains such as E. coli, S. aureus, P. aeruginosa and S. epidermidis. Antibacterial activity of various combinations of these three plant extracts reported for the first time in order to determine synergistic potential and pharmacological interactions. Ciprofloxacin served as positive control antibiotic. Zone of inhibitions of plant extracts was compared with standard antibiotic ciprofloxacin. Our findings reported that clinical strains E. coli and S. aureus that were completely resistant to Ciprofloxacin showed tremendous susceptibility towards separate and combined plant extracts. Most susceptible strain towards plant extracts was S. aureus followed by E. coli, S. epidermidis and P. aeruginosa. Most resistant strain towards plant extracts was P. aeruginosa. It was noted that prominent synergism occurred in most of the combined plant extracts highlighting medical importance for combating infections caused by multidrug resistant bacteria. Current study clearly suggests that various combinations of these three plant extracts could be used as potential natural resources in order to develop promising antibiotic for treating various infections.
Dr. Asma Waheed Qureshi has completed her PhD from University of The Punjab, Pakistan in Zoology. Currently she is working as Associate Professor and Head of The Department of Zoology, GC Women University Sialkot, Pakistan. She is also supervising PhD and MS research Scholars and more than 15 MS scholars has completed their degree under her supervision. She has also published more than 25 research articles in reputed journals and presented papers in national and international conferences. Her research focus are parasites and bacterial pathogens of animals and humans.
- Federal University of Ouro Preto, Brazil
- Title:Benznidazole, Itraconazole, and their Combination for the Treatment of Acute and Chronic Experimental Chagas Disease in Dog Model
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The association of other active drugs or substances with benznidazole (BZ) or nifurtimox (NF) has been stimulated to treat Chagas disease (CD) since these drugs present limitations. As the dog is an excellent model for Chagas disease study and its treatment, our study explored the association of BZ with the itraconazole (ITZ) in dogs infected with a Trypanosoma cruzi strain resistant to BZ and NF, treated at the acute and chronic phases of the infection. BZ+ITZ treatment reduced parasitemia, hemoculture, PCR positivity, cardiac inflammation, fibrosis, qPCR positivity, and mortality during the acute phase. During the chronic phase, a less severe reduction of the same parameters was observed, but the echocardiography revealed reduction on disease evolution. The pharmacokinetics of the Drug’s association was more advantageous for CD treatment than the monotherapy since benznidazole exposition was higher.
Marta de Lana: Brazilian, Emeritus Professor at the Federal University of Ouro Preto. Member of the Brazilian Academy of Pharmaceutical Sciences. Pharmacist, M.S., and Ph.D. in Parasitology at the Federal University of Minas Gerais. Postdoctoral program at the IRD, France. Professor of Clinical Parasitology at the Pharmacy School, UFOP since 1982, Director of the Pharmacy School and Head of Animal Care Facilities, UFOP. Recipient of a scholarship from the National Council for Scientific and Technological Development (CNPq). Advisor and professor in the Biological Sciences and Pharmaceutical Sciences Graduate programs, UFOP. Member of the Biological Sciences and Biotechnology and Member of the Health Sciences Committees, respectively, at the Foundation for Research Support (FAPEMIG). Institutional Coordinator of the State Network for Research on Pharmacological and Toxicological Tests of Drugs, Therapeutic Products, and Raw Materials, and the State Network for the Animal Care Facilities (FAPEMIG).
- Future University in Egypt, Egypt
- Title:Repositioning of Ticagrelor: Renoprotection Mediated by Modulating Renin-Angiotensin System, Inflammation, Autophagy and Galectin-3
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Acute kidney injury is a common ailment that ensues when the kidney is exposed to different insults, including ischemia/reperfusion (I/R), a disorder detected in cases of septic shock, cardiovascular surgery, and kidney transplantation and in addition it activates platelets to contribute more in renal injury. Ticagrelor, an antiplatelet that inhibits adenosine diphosphate (ADP)-mediated platelet aggregation via blocking of the purinergic receptor P2Y12, is known for its anti-ischemic cardioprotective capacity, but its renoprotective effect against I/R is rarely addressed, which is the aim of the current study.
Thus, adult male Wistar rats were allocated into 3 groups, namely sham-operated, I/R-operated (45 min/24 h) and I/R pretreated with Tica (30 mg/kg) for one week. Due to the scarce doses available in kidney injury models, a pilot study using 30 and 150 mg/kg of Tica was carried out and according to the obtained results, 30 mg/kg was chosen.
The pre-administration of Tica (30 mg/kg) for one week guarded against the harmful impact of I/R insult and improved renal histological structure and function, which was validated by the reduced cystatin-C, neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 and the classical markers, blood urea nitrogen (BUN) and creatinine. The renin-angiotensin system (RAS) plays a critical role in the kidney and in our study Tica has reverted the effect of I/R on the RAS related markers, where it downregulated the gene expression of the receptors prorenin and endothelin-1A and eventually led to a reduction in the potent vasoconstrictor angiotensin (Ang) II. On the other hand, Tica heightened the beneficial molecules, where it increased the activity of angiotensin-converting enzyme-2 (ACE-2) and the renal content of angiotensin 1-7 (Ang 1-7). Apart from the RAS, Tica signified its anti-inflammatory capacity by inhibiting the inflammatory transcription factor nuclear factor κB (NF-κB) and the surrogate inflammatory marker tumor necrosis factor (TNF)-α. Additionally, Tica enhanced the autophagy flux machinery that was hindered by the I/R insult. Here, Tica increased the protein expression of Beclin-1 and microtubule-associated protein light chain 3 II (LC-3 II) and abated the lysosomal marker cathepsin-D. Besides, Tica augmented cell survival by inhibiting caspase-3 activity and galectin-3, which is known as a marker of cardiac and renal injury.
Our study is the first to highlight the renal anti-ischemic potential of Tica via modulating the RAS, enhancing autophagy, and decreasing both inflammation and cell demise to improve renal morphology and function in a model of renal I/R.
I am Hanan Salah Eldin El-Abhar, Chair of the Pharmacology, Toxicology & Biochemistry department at Faculty of Pharmacy, Future University in Egypt (FUE; 2018-till now) and was Chair of the Pharmacology & Toxicology department at Faculty of Pharmacy, Cairo University for 6 years (2011-2017). I am a peer reviewer for several international journals in my specialty and I have 81 international published articles (1995-2022) and an Editor at the journal of “Integrative Diabetes and Cardiovascular Diseases” and “Future Journal of Pharmaceutical Sciences [FJPS])”. I am a member of several Scientific Associations, a member of the judging and discussion panel for many Masters and Ph.D. theses, and a member of the Masters and Ph.D. degrees equivalence department at the sector of specialized pharmaceutical studies in the Supreme Council of Universities in Egypt. I was a Consultant at the Pharmacology Committee of the Central Agency of Pharmaceutical Affairs (CAPA) at the Egyptian Ministry of Health (2013-2015) and Managerial Secretary and member of the permanent scientific committee for the promotion of assistant professors and professors at the National Organization for Drug Control and Research (NODCAR, 2013-2015). I am one of the World’s Top 2% Scientists list (Stanford University, 2021).
- Gopal Narayan Singh University, India
- Title:Antimicrobial Susceptibility Pattern of Bacterial Isolates from Intensive Care
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Background: Infection control is one of the major global public health problems due to rapid emergence of Anti-Microbial Resistance (AMR). Intensive Care Units (ICUs) are considered as epicenter of development of AMR. Management of diseases with various infections in the ICUs is a growing challenge for the health care providers. Updated understanding about the pathogen profile and antimicrobial susceptibility pattern has great importance in patients management and checking of AMR in ICUs.
Aim: Designing of preventive strategy against antimicrobial resistance by understanding the antimicrobial susceptibility pattern of bacterial isolates from the intensive care units of a tertiary care teaching health set up.
Material and Methods: A prospective study conducted from April, 2021 to June, 2022 in the Department of Microbiology NMCH Jamuhar, Sasaram. A total of 824 clinical specimens were collected from 231 patients out of 1784 patients admitted in the various ICUs of the hospital during the study period. Specimens were comprised of Urine, Blood, Endotracheal Secretions, Tissue, Pus swabs, Cerebrospinal fluid, Ascitic fluid, Peritoneal fluid, Pleural fluid, Catheter tip, Central Line tip and Vaginal swab. All bacterial isolates were identified following the standard microbiological and antimicrobial susceptibility testing was performed employing the disk diffusion technique as per Clinical and Laboratory Standards Institute (CLSI) guidelines 2021. Data analysis was done by using the Statistical Package for Social Sciences (SPSS), version 23 (IBM SPSS Statistics, Armonk, NY).
Results: In this study, 231 ICU admitted patients were included where there were 156 male and 75 female patients. A total of 824 specimens collected were composed of Urine 240 (29.1%), Blood 304 (36.8%) , Pus swab 107 (12.9%), Endotracheal secretions 90 (10.9%), Body fluids 30 (3.7%), Tissue 29 (3.5%), CSF 8 (1%) including Central line tip, Catheter tip and Vaginal swab 16 (1.9%). Maximum no of samples were obtained from the patients in the age between 21-40 years. Bacterial growth was observed in 659 (79.9%) out of 824 specimens while 165 (20.1%) showed no growth. Maximum isolates were found to be gram negative bacilli 384 (58.3%) where 275 (41.7%) were gram positive cocci. The study showed maximum isolation of Escherichia coli (ESBL+ Non ESBL) 143 (37.23%), followed by Klebsiella spp. 91 (23.6%), Pseudomonas aeruginosa 63 (16.4%), Acinetobacter spp. 36 (9.1%), Citrobacter spp. 30 (7.8%) and Proteus spp.13 (3.81%). Among Gram positive cocci, MRSA showed maximum growth 97 (35.3%), followe by S. aureus 93 (33.9%), Coagulase negative staphylococcus aureus 71 (25.6%) & Enterococcus spp 14 (5.2%). Candida spp. 8 (1.3%) were also isolated from various samples in the study. Anti-microbial susceptibility testing showed Colistin with highest (100%) sensitivity for E. coli, Pseudomonas spp. and Klebsiella spp. followed by Gentamicin (93%, 72%, & 52%) & Pip-tazo (84%, 78% & 71%) respectively. High antimicrobial resistance pattern was demonstrated against Tetracycline (81%, 53% & 79%), Ampicillin (69%, 48% & 71%) and Ceftazidime (100%, 56%, & 100%) for Pseudomonas spp., E coli and Klebsiella spp. respectively. Among Gram Positive isolates MRSA showed maximum sensitivity to Vancomycin and Linezolid (100%) followed by Gentamycin (97%) and Tecoplanin (84%) in the present study.
Conclusion: The study revealed that most of the isolated organisms were less sensitive to the 1st & 2nd line of antimicrobials with maximum sensitivity to the 3rd line of the same. It is an important evidence of emergence of multidrug resistant organisms in the health set up. This indicates further need of broad based hospital study with a larger sample size for more statistical conclusions to design the locally suitable surveillance strategy to prevent AMR as a part of global Antimicrobial Stewardship Program.
- Indian Institute of Technology Delhi, India
- Title:Biocontaminants as Potential CBRN Agents
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Contamination is the presence of unwanted material on the desirable surface. Bio-contamination is the undesirable presence of the microbes such as viruses, bacteria, and fungi or end products such as endotoxin and mycotoxins inside a medium and leads to a toxic effect on the living organisms. Since ancient times, microbial organisms have been used as potent biowarfare agents. From the battle of Tortola to Botulinum toxin in world war two, there was and is continuous use of the Biocontaminents as the Biological Warfare Agents or potential CBRN (chemical, biological, radiological, and nuclear) agent. The current Covid-19 crisis in the world puts a question mark concerning the preparation of the nations to mitigate natural disasters. There is an urgent requirement for research and innovation in mass decontamination in Biological emergencies.
Dr. Navneet Sharma completed his master’s in Pharmaceutics from the JSS University Mysuru and Ph.D. from DIPSAR University of Delhi at Defence Research and Development Organization (DRDO). His work was inclined more towards applied R&D, especially needs- based product development. He had devised and used pharmacoscintigraphic procedures for its preclinical evaluation of the drug delivery systems in various experimental models of disease. In collaboration with the Institute of Nuclear Medicine and Allied Sciences, Govt of India, he developed the dermal formulations for broad-spectrum decontamination. For the past four years, his work has been exclusively focused on the Medical Management of CBRN Emergencies. Currently, He is working as a scientist at IIT Delhi on the universal formulation for the CBRN decontamination of skin. The first quarter of the previous year motivated him to develop solutions for viral decontamination from living and inanimate surfaces. He was among the 4 Eminent Scientists from the country who got the fast track Covid-19 decontamination project from the Department of Sciences and Technology (DST). In his brief research carrier of 3 years, he had acclaimed eight national and international awards. The most prominent among them are the Shanghai Cooperation Organization Covid-19 best innovation award 2020 and the Department of Science and Technology Young Scientist Award- 2018. He had filled and granted ten national patents, and four technologies successfully transferred to the industry. He had 45 publications, including five book chapters, authored two books for Director-General Life Sciences DRDO, and Edited two books.
- Health Emergencies and Epidemics Control General Directorate, Sudan
- Title:First Report of Epidemic Dengue Fever and Malaria Co-Infections Among Internally Displaced Persons in Humanitarian Camps of North Darfur, Sudan
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Objectives: This study aimed to investigate an outbreak of a non-malaria, undifferentiated febrile illness, among internally displaced persons (IDPs) living in humanitarian camps in North Darfur, Sudan, in 2019.
Methods: An investigation team was deployed to North Darfur to identify suspected cases and collect blood samples, and clinical and demographical data. Blood samples were examined microscopically for Plasmodium spp and tested for dengue (DENV) and yellow fever viruses by reverse transcriptase-quantitative polymerase chain reaction.
Results: Between September 7 and December 18, 2019, we clinically identified 18 (24%), 41 (54%), and 17 (22%) cases of dengue fever, dengue with warning signs, and severe dengue, respectively. Blood samples were collected from 22% of patients, and 47% of these tested positive for DENV-1 RNA. We confirmed 32 malaria cases with 5 co-infections with DENV. This outbreak of dengue was the first among IDPs in the humanitarian camps.
Conclusions: Our findings indicate that dengue has become endemic or that there has been a new introduction. Further epidemiological, entomological, and phylogenetic studies are needed to understand disease transmission in the area. An early warning and response system and an effective health policy are crucial for preventing and controlling arboviruses in Sudan.
- Israel Institute of Technology, Israel
- Title:Myocarditis following COVID -19 vaccination
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In December 2020, the Ministry of Health in Israel approved the Pfizer-BioNTech mRNA vaccine (BNT162b2) for prevention of COVID-19 disease shortly after the Food and Drug Administration (FDA) issued an Emergency Use Authorization (EUA) for it. Shortly thereafter, during a three-week period between January 30th and February 20th 2021, six men were hospitalized in our department with myocarditis after receiving the vaccine. Five patients presented 24–72 hours after receiving the second dose of the vaccine and one patient presented 16 days after receiving the first dose of the vaccine. All patients were COVID-19 RT-PCR negative and non-reactive for anti-nucleocapsid protein antibodies. All patients responded to the vaccine as evidenced by being positive for antibodies against the spike protein. Further laboratory testing included a complete blood count, complete metabolic panel, troponin, C-reactive protein, and PCR testing and serological determination of antibodies against common infectious pathogens related to myocarditis. To the best of our knowledge, we were the first ones in the world to report the adverse reaction of myocarditis after BNT162b2 vaccination.
- General Manager of Gennova Biopharmaceuticals Ltd., Pune, India
- Title:Process for Large Scale Production of A Lead Malarial Vaccine Candidate (Pfrh5) in E Coli
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The Plasmodium falciparum (P. falciparum) reticulocyte binding protein homologue 5 (PfRH5) has recently shown great promise to be developed as a vaccine candidate to prevent blood stage malaria. Historically observed difference in the immunogenicity and efficacy profile of full-length PfRH5 antigen could also be attributed to its production methods, lack of characterization and structural integrity. To conduit this gap, methods have been developed to produce a well characterized full-length PfRH5 in E. coli expression system and further demonstrated its scalability up to 30 L fermenter scale. PfRH5 is refolded using DoE approach followed by a twostep chromatographic purification to get highly purified antigen. Thermal and fluorescence characterization of purified PfRH5 revealed its melting temperature and aggregation profile. Moreover, refolded and purified PfRH5 elicited high antibody titers while adjuvanted with GLA-SE during mice in vivo studies.
Dr. Arjun Raghuwanshi received his doctorate in Biotechnology from Pune University (SBPPU), India. He is currently employed as the General Manager of Gennova Biopharmaceuticals Ltd., Pune. He holds 18 years of rich experience in bio therapeutics, vaccines, process development and manufacturing operations. He worked for major Indian biopharmaceutical companies such as Wockhardt., Zenotech, Century, and Intas Pharmaceuticals. He developed various biosimilar products for the Indian and semi-regulated markets, including recombinant human Insulin, EPO, TNK-tPA, GCSF, PEG GCSF, Peg-Asparaginase, and Bevacizumab. He is currently working on a number of lead vaccine candidates in the fields of Malaria and Covid-19. To combat the ongoing pandemic, his team recently developed a SamRNA vaccine against Covid-19, which is now in phase II/III clinical trials in India. He holds two US process patents on recombinant GCSF (US 20180223270A1) and
TNK-tPA, (US20170210784A1) and also recently received an Indian patent on Bevacizumab ophthalmic composition, WO2021019576A1. In addition, he has published his research work in high repute journals and presented during various international conferences as invited speaker.
- University of California Irvine, USA
- Title:E-cigarette Vaping and COVID-19: Contributing Biological Factors Underlying Individual Differences
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Tobacco addiction remains one of the largest preventable causes of disease and death worldwide. Recently, the use of nicotine e-cigarettes has dramatically increased among many populations, including youth. These devastating trends have been paralleled by the increasing mortality rate due to the COVID-19 pandemic. With these infections, symptoms range from mild to severe. Cigarettes and other chemical constituents in vape solutions may induce inflammation and damage lung tissue, thereby potentially leading to more severe symptomology with viral infection. In lung tissue, angiotensin-converting enzyme 2 (ACE2) mechanistically underlies viral entry of COVID-19. In this talk, I will discuss our recent findings that have examined the effects of e-cigarette vapor inhalation on ACE2 and nicotinic acetylcholine receptor expression in male and female mice. Together, we reveal a mechanistic link between e-cigarette vaping and ACE2 expression, which supports the contention that nicotine vaping contributes to individual vulnerability for coronavirus infection.
Dr. Christie Fowler is an Associate Professor in the Department of Neurobiology and Behavior at the University of California Irvine. Her research aims to elucidate the neurobiological, epigenetic and extracellular signaling mechanisms underlying drug addiction. Dr. Fowler currently serves on the editorial boards for the Journal of Neuroscience, eNeuro and Neuropsychopharmacology, and on the executive committee for the Irvine Center for Addiction Neuroscience (ICAN). Of further note, Dr. Fowler received the highly prestigious Avenir Award from NIDA, and she was highlighted as a ‘Scientist to Watch’ by The Scientist magazine.
- Brighton and Sussex Medical School, United Kingdom
- Title:Systematic Review of Ethiopian Medicinal Plants Used For their Anti-Inflammatory and Wound Healing Activities
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Ethnopharmacological relevance: Plant materials are used worldwide as complementary and alternative therapeutics for the treatment of various illnesses. In Ethiopia, folk medicines are utilized across a wide range of cultures and settings. Ethiopia has numerous plant species of which around 12% are endemic, making it a rich source of medicinal plants that are potentially important for human wellbeing.
Aim of the study: The aim of this study was to assess Ethiopian medicinal plants with anti-inflammatory or wound healing activities, in an attempt to compile the information required for further investigation of their potential role in the management of lymphoedema.
Methods: A systematic review protocol was developed according to the preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) statement. The protocol for this review was registered on PROSPERO with registration number CRD42019127471. This review considers all controlled in vivo and in vitro anti-inflammatory and wound healing studies evaluating the efficacy and safety of Ethiopian medicinal plants. The search strategy included all articles containing descriptors such as Ethiopia, medicinal plants, herbal products, care, management, lymphoedema, lymphedema, swelling, podoconiosis, elephantiasis, wound, wound healing, inflammation, an anti-inflammatory that were published until June 28, 2019. Outcomes were measured as the percentage of inflammatory and pro-inflammatory cell inhibition, as the percentage of carrageenan-induced oedema (anti-inflammation) inhibition, and the percentage of cell migration and proliferation (wound healing). For quality assessment of individual animal studies, the Risk of Bias tool for animal intervention studies (SYRCLE’s RoB tool) criteria were used. For quality assessment of individual in vitro studies, the OECD guidelines and the WHO Good Laboratory Practice (GLP) handbook were used. Results: A total of 46 articles on anti-inflammatory and 17 articles on wound healing properties were reviewed. For the in vivo studies, Swiss albino mice and Wistar rats were used, and the concentration of plant extracts or fractions administered to the lab animals varied considerably. Acetone extract of Vernonia amygdalina (Delile) Sch.Bip. showed the fastest anti-inflammatory activity at lower concentrations in carrageenan-induced paw oedema. Conclusion: Lawsonia inermis, Azadirachta indica, Achyranthes aspera, and Cuminum cyminum are the most studied plant species in terms of anti-inflammatory activity, while Lawsonia inermis and Azadirachta indica are the most studied ones for wound healing. The most common in vivo techniques used for the anti-inflammatory and the wound healing assays were carrageenan-induced paw oedema, and excision and incision wound models, respectively.
Dereje Nigussie (MSc in Medical Pharmacology), Ph.D. Fellow, Brighton and Sussex Medical School, UK & CDT-Africa, Ethiopia
- University of Cambridge, United Kingdom
- Title:Cellulose Nanocrystals in Cancer Diagnostics and Treatment
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Cancer is currently a major threat to public health, being among the principal causes of death to the global population. With carcinogenesis mechanisms, cancer invasion, and metastasis remaining blurred, cancer diagnosis and novel drug delivery approaches should be developed urgently to enable management and treatment. A dream break-through would be a non-invasive instantaneous monitoring of cancer initiation and progression to fast-track diagnosis for timely specialist treatment decisions. These innovations would enhance the established treatment protocols, unlimited by evasive biological complexities during tumorigenesis. It is therefore contingent that emerging and future scientific technologies be equally biased towards such innovations by exploiting the apparent properties of new developments and materials especially nanomaterials. CNCs as nanomaterials have undisputable physical and excellent biological properties that enhanced their interest as biomedical materials. This article therefore highlights CNCs utility in cancer diagnosis and therapy. Their extraction, properties, modification, in- vivo/in-vitro medical applications, biocompatibility, challenges and future perspectives are precisely discussed.
Ishaq Lugoloobi (orcid No: 0000-0003-1640-6219) received his B.Sc., in Chemistry and Biology (major) and Education (minor) at Mbarara University of Science and Technology (MUST), Uganda, with support from the meritorious Uganda government undergraduate scholarship and emerged among the top best students at the university.
In 2018, he received an international Chinese government scholarship to continue his studies. In 2021, he graduated as a research student from Donghua University, Shanghai-China, pursuing M.Sc. Chemistry Engineering and Technology degree. His research focus is on the nanoengineering of biological and chemical molecules for chemical applications such as conductivity and biological applications such as drug delivery. He also has an interest in engineering pharmaceutical molecules.
- University Hospital Zagreb, Croatia
- Title:COVID-19 Convalescent Plasma in Immunodeficient Patients
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Since the first described case in December 2019, the coronavirus disease 2019 (COVID-19) has been one of the most significant challenges of our time. The clinical spectrum ranges from asymptomatic to critical forms with acute respiratory distress syndrome and death. The most endangered patients are the adults older than 60 years, the patients with comorbidities, especially immunodeficiencies. A targeted therapy and exact treatment protocols are lacking due to the not fully known pathogenesis. Passive immunotherapy with convalescent plasma transfusion represents a potentially effective therapy for some patients with impaired cellular and humoral immunity. This could be a long-term, ”chronic” therapy for COVID-19 for this group of patients, especially for bridging the period when the immune system cannot produce by itself the antibodies needed for viral clearance.
Dr. Dina Rnjak was born in Osijek, Croatia in 1989. She obtained her medical degree at Faculty of medicine, Josip Juraj Strossmayer University of Osijek. Her residency was at Special Hospital for Lung Diseases Zagreb and at The Clinic for Lung Diseases, University Hospital Zagreb. Dr. Rnjak is a member of the Croatian Thoracic Society, Croatian Resoiratory Society, European Respiratory Society, Croatian Medical Association.
- General Director of Allied Health Service, Palastine
- Title:COVID-19 in Eastern Mediterranean Region; Impact on Blood Supplies and Transfusion Services
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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spurred a global health crisis.The safety and supply of blood during this pandemic has been a concern of different blood banks and transfusion services as it is expected to reduce the blood supply and adversely affect blood system activities. We aim to assess the situation in the Eastern Mediterranean region (EMR) region during the first months of the pandemic. Methods: A survey was designed to address blood supply, transfusion demand and donor management during SARS-CoV-2 pandemic. Medical directors of different blood banks were invited to participate. Results: Total of 17 responses were received with representation from 15 countries in the region. 76% were from national blood banks. Most centers had a drop in the blood supply, ranging 10-75%. Representatives from 14 countries (93.3%) believed that public fear has contributed to decrease in donations. Most centers (n=12, 70%) had a reduction in transfusion demand, while those who didn’t reported heavy involvement in treating patients with underlying hemoglobinopathies and haematological malignancies. Half of the centers activated their emergency & contingency plans. Four centers had to alter the blood donor deferral criteria in order to meet demands. All centers implemented donor deferral criteria in relation to SARS-CoV-2, but were variable in other measures to mitigate the risk of donor and staff exposure. Conclusions: Blood services in the region faced variable degrees of blood shortages. Blood services need to take steps to plan, assess, and respond proportionately to future similar pandemics.
I am the General Director of Allied Health Services at the Palestinian Ministry of Health and the Presidant of the syndicate of the Palestinian Medical Technology Association. In addition, I am the President of the Arab Federation of Clinical Biology. I hold a Master degree in Medical Laboratory Sciences and a high diploma in Management for Middle Managers I am an astute manager, I led the capacity building of Lab. Medicine in Palestine particularly during the COVID-19 pandemic. I have several research activities and coauthored several publications.
- University of North Carolina, United States
- Title:Mycotoxins Causing Amyotrophic Lateral Sclerosis
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Amyotrophic Lateral Sclerosis(ALS) remains a terminal disease without an established etiology for the majority of patients. The dominant theory of ALS before the 1970’s was the presence of a poison. One of the primary means of treating patients with a toxic exposure has been plasma exchange, but plasma exchange of ALS patients failed to alter the clinical course. The failure of plasma exchange assumes the patient is no longer exposed to the poison. If the exposure to poisons continued, then plasma exchange alone would fail. I found laboratory evidence of a poisoning in every patient with ALS examined. A search for specific poisons found evidence of mycotoxins. Treatment with antifungal agents corrected the laboratory findings. All of the ALS patients had evidence of immune suppression. There is mounting evidence that many mycotoxins cause both neurotoxicity and immune suppression. These mycotoxins may be able to explain the full spectrum of pathology in ALS without a secondary event.
- Chengdu Second People’s Hospital, China
- Title:Burkholderia Gladioli Infection Isolated from the Blood Cultures of Newborns in the Neonatal Intensive Care Unit
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Burkholderia gladioli was described as a plant pathogen, and it is a rare cause of infection in humans that is primarily associated with human pulmonary infections, such as chronic granulomatous disease and cystic fibrosis. The neonatal respiratory system is not fully developed and cannot expel bacterial aerosol properly. A total of 2,676 newborns in the neonatal intensive care unit were retrospectively analysed in Putian City, Fujian Province, China, from 2011 to 2014. All of the blood samples were tested for C-reactive protein (CRP), procalcitonin (PCT) and white blood cell (WBC). B. gladioli infections were determined and analysed using a blood culture system. Antibiotic susceptibility testing was performed using the K-B method. Of the 2,676 participants, 87 (3.25 %) had a positive B. gladioli blood culture that occurred >72 h after birth, including a premature group (54.0 %, asphyxia [vs. 9.20 %], fever [vs. 13.80 %], pneumonia [vs. 6.90 %] and hyperbilirubinaemia [vs. 8.05 %]) and newborns with necrotising enterocolitis (NEC) (vs. 5.75 %). The mean±standard deviation (SD) of the CRP level was 12.31±0.26 mg/L and that of the PCT level was 1.53±0.21 ng/ml in the 87 B. gladioli-infected newborns. Most of the B. gladioli isolates were sensitive to many antimicrobial agents and did not lead to serious consequences. All of the B. gladioli-infected newborns were unhealthy, especially the premature infants. B. gladioli might be a causative bacteraemia agent in neonates, it appears to have pathogenic potential in newborns and its sensitivity to antibiotics may be a beneficial factor.
Zhou fangye, female, graduated from pathogenic biology of Peking Union Medical College in 2012, and now works in Chengdu Second People’s hospital. He mainly engaged in the research of pathogenic biology and infectious diseases, published 5 SCI papers and presided over 3 scientific research projects.
- Al-Baydha University, Yemen.
- Title:Targeting Hif-1α by Newly Synthesized Indolephenoxyacetamide (Ipa) Analogs to Induce Anti-Angiogenesis Mediated Solid Tumor Suppression
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Background: Hypoxic microenvironment is a common feature of solid tumors, which leads to the promotion of cancer. The transcription factor, HIF-1α, expressed under hypoxic conditions stimulates tumor angiogenesis, favoring HIF-1α as a promising anticancer agent. On other hand, synthetic Indolephenoxyacetamide derivatives are known for their pharmacological potentiality. With this background here, we have synthesized, characterized, and validated the new IPA (8a-n) analogs for anti-tumor activity. Methods: The new series of IPA (8a-n) were synthesized through a multi-step reaction sequence and characterized based on the different spectroscopic analysis FT-IR, 1H, 13C NMR, mass spectra, and elemental analyses. Cell-based screening of IPA (8a-n) was assessed by MTT assay. Anti-angiogenic efficacy of IPA (8k) validated through CAM, Rat corneal, tube formation and migration assay. The underlying molecular mechanism is validated through zymogram and IB studies. The in-vivo anti-tumor activity was measured in the DLA solid tumor model. Results: Screening for anti-proliferative studies inferred, IPA (8k) is a lead molecule with an IC50 value of ˜5 μM. Anti-angiogenic assays revealed the angiopreventive activity through inhibition of HIF-1α and modulation downstream regulatory genes, VEGF, MMPs, and P53. The results are confirmative in an in-vivo solid tumor model. Conclusion: The IPA (8k) is a potent anti-proliferative molecule with anti-angiogenic activity and specifically targets HIF1α, thereby modulates its downstream regulatory genes both in-vitro and in-vivo. The study provides scope for new target-specific drug development against HIF-1α for the treatment of solid tumors.
Fares Hezam Al-Ostoot, Obtained his MSc at SRTM University in 2015 goting first class with distinction, and he is a Ph.D. research scholar, currently works at the Department of Medical Biochemistry, Mysore University, Mysuru, India. He joined as an assistant professor at Al-Baydha University, Yemen in 2008. He is an academic lecturer in the Department of Biochemistry, Faculty of Education and Science, University of Al-Baydaah, Yemen. He has published more than 34 research articles in reputed journals.
- Jamia Millia Islamia University, New Delhi
- Title:Natural Compounds of Amaranthaceae: As a Novel Potentiate Factor Against Mtb Infection
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Mycobacterium tuberculosis (Mtb) is a bacterium that is difficult to defeat. It is one of the main causes of morbidity and mortality and is the primary cause of Tuberculosis (TB). Elective curative methodologies are increasingly securing drug hindrance for TB requests. The use of medicinal plants in the treatment of TB to fortify chemotherapy regimens has been studied. The study presented was carried out to investigate the efficacy of the family plant Amaranthaceae against Invitro and Insilico anti-tuberculosis activity. Plant extracts were prepared in various solvents from aerial and root components tested for total phenolic and flavonoid content (TPC & TFC). Minimum inhibitory concentration (MIC) against various strains of MTB determined the effectiveness of plant extract and the higher MIC extract was studied for phytoconstituents. Their targeted Mtb proteins were characterized by molecular docking and clarifying the mechanism behind bacterial growth inhibition. The targeted bacterial protein will be characterized biochemically and biophysically. Amaranthaceae plant methanol extract was successively fractionated in different solvents and the ethyl acetate faction showed high TPC and TFC concentrations and maximum anti-tuberculosis activity in (ATCC 27294) strain. Molecular docking evaluated the top 10 targeted bacterial proteins with binding affinity (>-8.5 Kcal/mol) with respective phytoconstituents. In biophysical activities therefore the selected compound has showed the significant outcome for utilize in antituberculosis approaches. The study accomplishes that Ethyl acetate exhibited promising antituberculosis activity and can further utilize in pharmacology field for effective treatment against TB. Targeted protein of the identified compounds was determined which will be helpful in further accentuate their mechanism of action in virulence.
Md Amjad Beg is a PhD student in CIRBSc, Jamia Millia Islamia University New Delhi, India. He has 16 publication which is in peer reviewed journals. His scientific interests focus on the field of Medicinal Chemistry, Microbiology, Molecular Biology and Computational Biology. His main objectives are to understand the working mechanism and effectiveness of plant extract and natural compounds over Mycobacterium (MTB). Due to the limitation of a drug for this malady, this study might be a beneficial step towards understanding the impact of these proteins and phytochemicals over bacterium.
- University Paulista, Brazil
- Title:Computational Search for Drug Repurposing to Identify Potential Inhibitors against SARS-COV-2 using Molecular Docking, QTAIM and IQA Methods in Viral Spike Protein – Human ACE2 Interface
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With the advancement of the Covid-19 pandemic, this work aims to find molecules that can inhibit the attraction between the Spike proteins of the SARS-COV-2 virus and human ACE2. The results of molecular docking positioned four molecules at the interaction site Tyr-491(Spike)-Glu-37(ACE2) and one at the site Gly-488(Spike)-Lys-353(ACE2). The QTAIM and IQA data showed that the 1629 molecule had a significant inhibitory effect on the Gly488-Ly353 site, decreasing the Laplacian of the electronic density of the BCP O4 -N10 . The molecule 2542 showed an inhibitory effect in two regions of interaction of the Tyr491-Glu37 site, acting on the BCPs H30-H33 and O8-H31 while the ligand 2600, in conformation 26, presented a similar effect only on the BCP O8-H31 of that same interactive site. Thus, the data suggest laboratory tests of a combination of molecules that can act at two sites of interaction simultaneously, using the combination of 1629/2542 and 1629/2600 ligands.
Professor Sergio Henrique Dias Marques Faria received a bachelor’s degree in Chemistry at the State University of Campinas (UNICAMP-BRAZIL). Faria did a Master and Doctorate in Theoretical Chemistry applied to Spectroscopy (UNICAMP). As Titular Professor at Universidade Paulista, Faria worked on the application of Molecular Docking calculations combined with electronic density (DFT) to quantify the interaction of antineoplastic agents with DNA fragments to clarify chemical mechanisms and new drug developments. These methods were used to develop drugs to inhibit SARS-COV-2 infection. Currently, Faria also works as a Postdoctoral Researcher at the University of São Paulo with atomic partition models for the study of singlet oxygen formation through photosensitizers.
- University of Naples Federico II , Italy
- Title:CRT Implantation after TLE in a Patient with COVID-19: Endocarditis Triggered by SARS-COV-2 Infection? A Case Report
- Time :
In the era of coronavirus disease 2019 (COVID-19), the management of cardiac implantable electronic devices infections with concomitant viral infection has not been completely defined yet. In this explorable context, we report the first experience of a Cardiac resynchronization therapy with defibrillator (CRT-D) implantation after transvenous lead extraction for endocarditis in a COVID-19 patient. We describe both the measures and procedures implemented to reduce the cross-infection in the operating room and our clinical practice to improving procedure effectiveness on patient care.
Graduated from the Faculty of Medicine and Surgery at the University of Naples “Federico II” and then further specialised in Cardiology. Dr. Stefano De Vivo for 30 years has taken care of his patients with dedication and passion. As a cardiologist he keeps himself updated with all new developments, specialising in Arrhythmology and diseases of the cardiovascular system. Today he is a first level manager at the “Azienda Ospedaliera dei Colli” in Naples where he has obtained the function for the management and extraction of CIED (cardiac implantable electronic devices).
- University Agostinho Neto, Luanda-Angola.
- Title:May Vital Signs And Biochemical Markers Be Easy Predictors Of Malaria Outcomes? Results Of An Observational Study In Angola
- Time :
Background: the search for predictors of malaria outcome is essential, because a successful, inexpensive, fast and easy to measure predictive test, with minimal infrastructure requirements or specialized training, can be used in malaria endemic environments to reduce serious outcomes and reducing the impact of the disease in poor countries like Angola. Objective: analyze whether vital signs and biochemical markers can be easily predictors of malaria outcome. Methodology: the study was a cross-sectional study and quantitative approach in 194 patients hospitalized for malaria evaluated in five of the seven days of follow-up. Descriptive statistics were computed using the SPSS v20.0 statistical programs and graphs in the Sigmaplot 12.0. Results: of the 194 patients followed, 68 were discharged, 22 died and 104 remained hospitalized after 7 days of follow-up, the mean parasitemia value was higher among patients whose outcome was death (2,909 p/mm3, SD=45) when compared to patients who were discharged (2,258 p/mm3, SD=3,301) and patients who remained hospitalized at the end of the study (1,269, SD=1,557), the mean of parasitemia at the end of the study was less than 60 p/mm3. The highest mean of creatinine was observed in patients who died both on admission (3.7 mg/dL, SD=1.8), compared to patients who remained in hospital (1.8 mg/dL, SD=2.3) and patients were discharged from the hospital (1.5 mg/dL, SD=1.9), however, although there were some changes over the follow-up period, the final mean creatinine of the study was greater than 1.6 mg/dL in all groups and the highest among those who had died (2.8 mg/dL, SD=1.8). Patients who died had a higher mean urea value at admission (78.3 mg/dL, SD=54.9), compared with patients who had been hospitalized (52.3 mg/dL, SD=41.7) and patients were discharged from hospital (48.1 mg/dL, SD=44.9), however, although there were some changes over the follow-up period, the final mean urea in all groups was greater than 44 mg/dL in all groups, especially in patients, died (74 mg/dL, SD=56). The highest mean temperature value was observed in patients who died (37.7ºC, SD = 1.7), compared to the temperature of hospitalized patients (37ºC, SD = 1.2) and patients who were discharged from hospital (36.8ºC , SD = 1.2), at the end of the study, the final mean temperature in all groups was above 36.6 ºC. Another vital sign that presented a higher mean of greater value in patients who died were respiratory cycles (22.7 cycles/min, SD=3.7), compared to the temperature of hospitalized patients (21.6 cycles/min, SD=4.1) and patients discharged from hospital (21.2 cycles/min, SD=3.5), at the end of the follow-up the final average of respiratory cycles in all groups was greater than 21.1 cycles/min. The mean weight of patients who were discharged from death was the highest observed in the study (69 kg, SD = 13), when compared to patients who were discharged and who were hospitalized (less than 64.7 kg, SD less than 11.8), at the end of the follow-up, the patients average weight did not change.The highest mean systolic blood pressure value was observed in patients who remained hospitalized (140.9 mmHg, SD=37), however patients who were discharged and patients who died, had similar mean systolic pressure (133.2 mmHg, SD=34) and at the end of the follow-up, the mean systolic pressure in all groups was less than 128 mmHg. Diastolic pressure did not show a big difference between the three groups of patients followed (less than 83 mmHg, SD less than 29) in patients who were discharged, who died, and who remained hospitalized, at the end of the follow-up, the mean pressure diastolic in all groups was less than 79.4 mmHg. The mean oxygen saturation value also did not show much difference between patients who were discharged, who died and who remained hospitalized (greater than 98%, SD less than 3.2), at the end of the follow-up the average desaturation of oxygen in all groups remained above 98.2%. Conclusions: markers such as parasitemia, urea and creatinine, associated with vital signs such as temperature, pulse, stroke cycles, weight and systolic blood pressure seem to be predictors of outcomes in the population studied, especially on admission, however they vary over time and therefore there are needs for further studies to assess the extent to which these predictors are important signs of outcomes. Vital signs such as temperature, pulse, breathing cycles and systolic blood pressure were shown to be initial predictors of outcomes and biochemical markers such as urea and creatinine appeared to be permanent predictors of outcomes, which is why poor countries like Angola where in most hospital units do not offer conditions for carrying out laboratory tests, the use of predictors such as these can make a difference in the medical and medication approach and reduce the impact of malaria on patients’ lives.
Keywords: I. vital signs II. biochemical markers III. easily predictors IV. malaria outcome.
PhD in Health Sciences by the Graduate Program of the School of Medicine of PONTIFÍCIA UNIVERSIDADE CATÓLICA DO PARANÁ (PUCPR) in 2017. Master’s Degree in Biochemistry and Molecular Biology by the Department of Biochemistry and Molecular Biology at the School of Biological Sciences and Health UNIVERSITY FEDERAL DO PARANÁ (UFPR) in 2014. Postgraduate (Specialization) in Clinical and General Microbiology by the School of Biosciences at PONTIFÍCIA UNIVERSIDADE CATÓLICA DO PARANÁ (PUCPR) in 2012. Postgraduate (Specialization) in Health Surveillance by the FACULDADE DE CIENCES DE WENCESLAU BRAZ (FACIBRA) in 2017. Graduation in Nursing from the SUPERIOR HEALTH SCIENCES INSTITUTE of UNIVERSITY AGOSTINHO NETO (ISCISA/UAN) in 2010. Graduation in Pedagogy from the FACULTY OF SCIENCES OF WENCESLAU BRAZ (FACIBRA) in 2017. He is post-doctoral student in Health Sciences at the School of Medicine of PONTIFÍCIA UNIVERSIDADE CATÓLICA DO PARANÁ (PUCPR). He is currently a professor at the INSTITUTO SUPERIOR DE HEALTH SCIENCES/UNIVERSIDADE AGOSTINHO NETO. He has experience in the area of General Nursing, with an emphasis on Urgent and Emergency Care, Public Health and Intensive Care. He has experience in service administration and head of nursing teams, especially in Urgent and Emergency Care and has also worked in intensive care. He has experience in Biochemistry and Molecular Biology and General Microbiology, working mainly in teaching, laboratory research and hospital care. He works with research projects focused on comorbidity and mortality in malaria, Acute Kidney Injury in malaria, CKD, Polymorphism of blood groups and susceptibility to malaria, sickle cell anemia and malaria and other projects.
- Imam Abdulrahman Bin Faisal University, Saudi Arabia
- Title:Imaging Differences between Coronavirus Disease 2019, Severe Acute Respiratory Syndrome, and Middle East Respiratory Syndrome
- Time :
Since the outbreak of Coronavirus Disease-19 (COVID-19) infection in December 2019 in Wuhan, the capital Hubei province, central of China, more than 4 million people have contracted the virus worldwide. Despite the imposed precautions, coronavirus disease-19 is rapidly spreading with human-to-human transmission resulting in more than 290,000 death as of May 13, 2020 according to World Health Organization (WHO). The aim of this study was to revise the characteristic imaging features of Sever Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS) during their outbreak, and to compare them with that of COVID- 19, to familiarize radiologists with the imaging spectrum of corona-virus syndromes. This study will help in more understanding and characterisation of COVID-19 to support the global efforts in combating its worldwide outbreak.
Dr. Osama Abdalla Mabrouk Kheiralla, Consultant Radiologist, Assistant Professor of Radiology – Radiological Sciences Department – College of Applied Medical Sciences – Imam Abdulrahman bin Faisal University – KSA. I am very interested in academic activities, especially research studies. I am very talented in conveying information and have very good ability to create good bonds with my students. I have supervised many research projects done by students’ groups. I have very wide experience in all radiological aspects including MRI and Spiral CT as well as all Ultrasound investigations. I have excellent experience in non-vascular intervention procedures like drain and biopsy from different body parts and nephrostomy tube insertion as well as ultrasound guided biopsy and CT guided biopsy. I believe that that learning is a process that never ends, and the good practitioner must never stop learning and should always try to keep on improving. Teaching is full of surprises and new situations, and one must be ready to adapt and discover new strategies of teaching. What remains true is the importance of understanding how to relate one’s knowledge to daily life practice.
- Galgotias University, India
- Title:Gel–Liquid Crystal Phase Transition and the Dynamics of Liposomes in Aqueous Solution
- Time :
In aqueous media, phospholipids are self-assembled into lipid bilayers. In biological membranes, proteins are hidden in lipid bilayers. It is well known that each protein and lipid have distinct characters. From the structures and functions study, it is revealed that different types of intermolecular interactions, viz. H-bond, van der Waals, hydrophobic, hydrophilic interactions play a significant role in the ability of these molecules. The DSC and dielectric spectroscopic techniques were used to study the gel-liquid crystal phase transition and the dynamics of DPPC molecules in aqueous media. The gel-gel pre-transition and gel-liquid crystal main transition were found from DSC study are strongly supported by x-ray measurement studied by Janiak et al. [Biochemistry, 15, 4575 (1976)] and present dielectric study from the temperature-dependent dielectric relaxation strength, relaxation time and symmetric shape parameter of the relaxation functions. From the complex dielectric study, four relaxation processes were observed from 40 Hz to 30 GHz frequency, which were ascribed to different molecular mechanisms, related to the structural units of the system. Surprisingly, relaxation process 3 observed near 10 MHz region due to motion of ions, which are related to the molecular motion of the lipid makes the bridge between lipid membrane and non-biological systems like ferroelectric liquid crystals. The process 3 is analogous to the variation of the soft mode observed in ferroelectric liquid crystals which was detected close to smectic-C*–smectic-A phase transition region. It is also interesting to find some physical properties of lipids such as depression of melting point, change of bilayer thickness, effect of domain structure, pH, etc. are strongly influenced by anesthetic molecules.
Dr. Shyamal Kumar Kundu is a Professor and Division chair in the Department of Physics, Galgotias University, Uttar Pradesh, India. Prior to that, he received Ph.D. degree from Indian Association for the Cultivation of Science, Kolkata, India. He was a postdoc at NCTU, Taiwan 2005, JSPS postdoc at Tokai University, Japan (2005-2007), Research Associate at Neutron Science Laboratory, The University of Tokyo, Japan (2007-2009), Research Scientist at the Jülich Center for Neutron Science (ICS-1), Forschungszentrum Jülich, Germany (2009-2012). He has received “Young Scientist Award” by Materials Research Society, Kolkata, India, “JSPS award” by JSPS, the Government of Japan and also “Bharat Ratna Dr. Abdul Kalam Gold Medal Award” by Global Economic Progress and Research Association, India. His scientific interests focus on the physical properties and the molecular dynamics of complex molecular systems like biomaterials, liquid crystals, gels, star like colloids and micelles. His expertise areas are Broadband Dielectric Spectroscopy, Rheology, Neutron Scattering and Light Scattering.
- Shahid Beheshti University of Medical Sciences, Iran
- Title:Efficacy and Safety of Proposed Bevacizumab Biosimilar BE1040V in Patients with Metastatic Colorectal Cancer: A Phase III, Randomized, Double-blind, Noninferiority Clinical Trial
- Time :
The purpose of this study was to compare the efficacy and safety of a proposed bevacizumab biosimilar to those of the reference
product in patients with metastatic colorectal cancer (mCRC).
This Phase III, multicenter, randomized, double-blind (patient- and assessor-blind), active controlled, 2-armed, parallel group, noninferiority trial was conducted in patients with histologically verified colorectal cancer with evidence of at least 1 metastasis. Patients with mCRC were randomized 2:1 to receive 5 mg/kg IV of either study drug plus FOLFIRI-3 (with repeated irinotecan 100 mg/m2
60-min infusion on day 3) or the reference drug plus FOLFIRI-3 every 2 weeks for 1 year. Progression-free survival (PFS) was the primary end point, and overall survival, objective response rate, and time to treatment failure as well as safety and immunogenicity were
secondary end points. The population assessable for PFS was per protocol, and the intention-to-treat population was used for sensitivity
analysis. Safety was assessed based on reports of adverse events, laboratory test results, and vital sign measurements.
A total of 126 patients were enrolled; PFS values in the biosimilar and reference arms were 232 days (7.7 months) and 210
days (7 months), respectively (P = 0.47). The hazard ratio of the biosimilar arm versus the reference arm was 0.79 in the per-protocol
population (90% CI, 0.46-1.35; P = 0.47). The upper limit for the 2- sided 90% CI was lower than the margin of 1.44, indicating that the
biosimilar drug was noninferior to the reference drug. The hazard ratio for overall survival in the intent-to-treat population was 0.99 (95% CI, 0.55-1.80; P = 0.99). The difference between other efficacy end points among the groups was not statistically significant. No significant difference was observed in the comparison of the two arms for safety. The antidrug antibody was positive in 1 patient in each arm.
The proposed biosimilar BE1040V was noninferior to the reference product in terms of efficacy in the treatment of mCRC, and
tolerability was comparable between the 2 drugs.
Doctor Sina Salari was born in Tehran in 1973 and got accepted to Isfahan Medical University in 2000. In 2007 he completed his specialty
training in Internal Medicine at Tehran University and completed his 3 years fellowship in Hematology and Oncology at Shahid Beheshti
Medical University, graduating with distinction in 2012. He was appointed as “Assistant Professor” and “Faculty member” in 2013. His
responsibilities from 2013 to 2020 include the followings: Director of the deputy of treatment of Oncology-Hematology and BMT department, Physician in charge of the international patients’ department (IPD), Head of the internal department and responsible
authorized physician of Ayatollah Taleghani Hospital. Some of his achievements in his field are as below: Presenting variety of lectures
in national seminars, publishing more than 30 research articles in national and international journals, Attaining ESMO, ASCO, ASBMT,
and APBMT membership. Dr.Salari has a particular interest in gastrointestinal and breast cancer. He has also widely researched in
lymphoma and multiple myeloma treatments and Cell therapy. He is member of the Cancer-Immunotherapy scientific committee and
organized the first cancer Immunotherapy Congress in Shiraz in 2019.
Dr.Salari performed the first research on the Monoclonal Antibody drug “Rituximab” for treating patients with lymphoma in Iran in 2011.
He is also involved in most studies related to Biosimilar medicines for cancers, collaborating with Iran Food and drug administration as a
consultant. He wrote the first RCT paper on the biosimilar drug of “Stivant” which was published in the Clinical Therapeutics journal in
- Sao Paulo University, Brazil
- Title:Evidence of Genomic Information and Structural Restrictions of HIV-1 PR and RT Gene Regions from Individuals Experiencing Antiretroviral Virologic Failure
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Objectives: This study analyzed Protease-PR and Reverse Transcriptase-RT HIV-1 genomic information entropy metrics among patients under antiretroviral virologic failure, according to the numbers of virologic failures or resistance mutations.
Methods: For this purpose, we used genomic sequences from PR and RT of HIV-1 from a cohort of chronic patients followed up at Sao Paulo Hospital. Results: Informational entropy proportionally increases with the number of antiretroviral virologic failures in PR and RT (p < .001). Affected regions of PR were related to catalytic and structural functions, such as Fulcrum (K20) Flap (M46) and Cantilever (A71). In RT, this occurred at Fingers (E44) and Palm (K219). Informational entropy increases according to the number of resistance mutations in PR and RT (p < .001). Higher PR entropy was proportional to the resistance mutation numbers in Fulcrum (L10), Active site (L24) Flap (M46), Cantilever (L63) and near Interface (L90). In RT, they related to regions responsible for protein stability such as Fingers (T39) and Palm (L100). Conclusions: The antiretroviral selective pressure affects HIV genomic informational entropy at the PR and RT regions, leading to the emergence of more unstable virions. Mapping the three-dimensional structure in these HIV-1 proteins is relevant to designing new antiretroviral targeting resistant strains. Biography
Research Interests: My research line involves the analysis of genomic signatures evolution of molecules and specific sites and epitopes selection and recognition for development of vaccines efficiency and anti-HIV drugs. I´m very motivated and interested in various Science projects and research experiments. I´m currently working on projects that involves the genome viral evolution and nanotechnology to HIV vaccine at University of Sao Paulo and Infectious Diseases Division, Department of Medicine, Federal University of São Paulo in collaboration to Telecommunications and Control Engineering Department, Engineering School, both in Brazil.