Abstract

The ethnic communities of Darjeeling hills still depend on plant-derived resources for disease cure. Although folk medicine is perhaps the most popular in tribal area, it has not been explored fully till now. In recent years, bioactive plant constituents have got much attention for the development of new therapeutic agents. A wide variety of phytochemicals are being screened for their anticancer potential. Natural products from medicinal plants, either as pure compounds or standardized extracts provide indefinite prospects for new drug leads because of the unmatched availability of chemical diversity. Modernization of ethnomedicinal plant remedies through standardization and quality control is a key factor that will govern their widespread acceptance by the international community. We had screened more than 30 medicinal plants of Darjeeling district area, which are used by ethno-practitioner for different disease cure, and tested for anticancer activity against three cancer cell lines HeLa, HepG2, and MCF 7. The anticancer activity was determined by MTT assay, LDH detection and Trypan blue dye exclusion assay. Maesa macrophylla, showed a highly promising cytotoxic activity against HeLa (IC50 9.55 μg/ml) and MCF7 (IC50 16.19 μg/ml) cell lines. Phytochemical analysis revealed the presence of multiple medicinally active compounds. We have found promising anticancer property in ethanolic leaves extracts of Maesa macrophylla and outcome was published in Bio Med Central (BMC) complementary and alternative medicine journal. Our next goal was to identify and isolate bio-active natural compound which was responsible for anticancer activity. The ethanol extracts were further fractioned and tested for anticancer activity and analyzed by GCMS. The beta-amyrone, triterpenoid, is a bio-active compound from Masea macrophylla could have anticancer property. In silico data suggested that beta-amyrone could have interaction with cytochrome P450. Cytochrome p450 play a dominant role in the metabolism of carcinogens and drugs that mitigate cancer growth. It also contributes in diversification of plant metabolites and play important role in triterpenoid biosynthesis. Bioactive compound, beta-amyrone, could be a hope of cancer treatment in future.

Biography

Myself, Dr. Anoop Kumar, born and brought up in Meerut, Uttar Pradesh, India. After completing my under graduation, I shifted to University of Allahabad, India for my post graduation in Biotechnology. I have completed my Ph.D. from School of Biotechnology, Jawaharlal Nehru University (JNU), New Delhi, India. I joined Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India for research work. I joined the University of North Bengal, Darjeeling, West Bengal, India and established my own cell culture laboratory ANMOL (Advanced Nanoscale Molecular Oncology Laboratory) and published a number of paper in reputed journals in collaboration.

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