Abstract

Mycobacterium tuberculosis (Mtb) is a bacterium that is difficult to defeat. It is one of the main causes of morbidity and mortality and is the primary cause of Tuberculosis (TB). Elective curative methodologies are increasingly securing drug hindrance for TB requests. The use of medicinal plants in the treatment of TB to fortify chemotherapy regimens has been studied. The study presented was carried out to investigate the efficacy of the family plant Amaranthaceae against Invitro and Insilico anti-tuberculosis activity. Plant extracts were prepared in various solvents from aerial and root components tested for total phenolic and flavonoid content (TPC & TFC). Minimum inhibitory concentration (MIC) against various strains of MTB determined the effectiveness of plant extract and the higher MIC extract was studied for phytoconstituents. Their targeted Mtb proteins were characterized by molecular docking and clarifying the mechanism behind bacterial growth inhibition. The targeted bacterial protein will be characterized biochemically and biophysically. Amaranthaceae plant methanol extract was successively fractionated in different solvents and the ethyl acetate faction showed high TPC and TFC concentrations and maximum anti-tuberculosis activity in (ATCC 27294) strain. Molecular docking evaluated the top 10 targeted bacterial proteins with binding affinity (>-8.5 Kcal/mol) with respective phytoconstituents. In biophysical activities therefore the selected compound has showed the significant outcome for utilize in antituberculosis approaches. The study accomplishes that Ethyl acetate exhibited promising antituberculosis activity and can further utilize in pharmacology field for effective treatment against TB. Targeted protein of the identified compounds was determined which will be helpful in further accentuate their mechanism of action in virulence.

Biography

Md Amjad Beg is a PhD student in CIRBSc, Jamia Millia Islamia University New Delhi, India. He has 16 publication which is in peer reviewed journals. His scientific interests focus on the field of Medicinal Chemistry, Microbiology, Molecular Biology and Computational Biology. His main objectives are to understand the working mechanism and effectiveness of plant extract and natural compounds over Mycobacterium (MTB). Due to the limitation of a drug for this malady, this study might be a beneficial step towards understanding the impact of these proteins and phytochemicals over bacterium.

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